Supplementary Figure 11: Comparison of analysis speed of TwoTone and iSMS. | Nature Methods

Supplementary Figure 11: Comparison of analysis speed of TwoTone and iSMS.

From: iSMS: single-molecule FRET microscopy software

Supplementary Figure 11

(a,b) Screenshots of the (a) TwoTone and (b) iSMS main windows. (c) Comparison of computational processing times for extraction of FRET traces from the same raw movie data set. In total, iSMS performed 17 times faster than TwoTone for this data set. TwoTone found 185 molecules and iSMS found 241 molecules. TwoTone software: Holden, S. J. et al. Biophysical Journal 99, 3102–3111 (2010)

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