Supplementary Figure 1: Aligning donor and acceptor emission channels in smFRET using iSMS. | Nature Methods

Supplementary Figure 1: Aligning donor and acceptor emission channels in smFRET using iSMS.

From: iSMS: single-molecule FRET microscopy software

Supplementary Figure 1

The figure shows an image registration between donor (green) and acceptor (red) emission channels. (a) The algorithm starts with an initial guess and (b) returns the positions of the aligned ROIs. The black inserts are overlay images in which the green color channel is the donor ROI and the red color channel is the acceptor ROI image data. The raw image frame is of size 512x512 pixels in this example.

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