Isolation of nuclei in an isotonic buffer retains chromosome loops and allows the probing of intrinsic loop conformation.
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References
Brant, L. et al. Exploiting native forces to capture chromosome conformation in mammalian cell nuclei. Mol. Syst. Biol. 12, 891 (2016).
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Rusk, N. Native chromosome conformation. Nat Methods 14, 105 (2017). https://doi.org/10.1038/nmeth.4172
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DOI: https://doi.org/10.1038/nmeth.4172
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