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Focus on next-generation sequencing data analysis

Nature Methods volume 6, page S1 (2009)Cite this article

What used to take years and extensive collaborations—generating the raw sequence of the three gigabases in the human genome—can now be done in a few days by a single investigator using a single run on some of the latest next-generation sequencing machines. The drawback is that this massive amount of data comes in the form of short reads, and one needs to invest heavily in computational analysis and choose from a plethora of tools to make sense of it all.

The recurring theme when it comes to the choice of software is that a 'one-size-fits-all' program does not exist, but users have to mix and match, which requires knowledge about the analysis steps in a given application and how different software operates at each step. This Focus aims to guide readers in their choice of software so they can extract a maximum of information from the data.

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  1. Nicole Rusk
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Rusk, N. Focus on next-generation sequencing data analysis. Nat Methods 6 (Suppl 11), S1 (2009). https://doi.org/10.1038/nmeth.f.271

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