With the realization that cells interact extensively with their surrounding microenvironments during growth and development, the challenge for researchers has become designing three-dimensional culture systems that more closely mimic those relationships.
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References
Engler, J.A., Sen, S., Sweeney, H.L. & Discher, D.E. Matrix elasticity direct stem cell lineage specification. Cell 126, 677–689 (2006).
Adamo, L. et al. Biomechanical forces promote embryonic haematopoiesis. Nature 459, 1131–1134 (2009).
North, T.E. et al. Hematopoietic stem cell development is dependent on blood flow. Cell 137, 736–748 (2009).
Lutolf, M.P. & Hubbel, J.A. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat. Biotechnol. 23, 47–55 (2005).
Kloxin, A.M., Kasko, A.M., Salinas, C.N., & Anseth, K.S. Photodegradable hydrogels for dynamic tuning of physical and chemical properties. Science 324, 59–63 (2009).
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Blow, N. Cell culture: building a better matrix. Nat Methods 6, 619–622 (2009). https://doi.org/10.1038/nmeth0809-619
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DOI: https://doi.org/10.1038/nmeth0809-619
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