PCR—the workhorse of modern molecular biology—is charging forward using both conventional and digital methods to explore single cells and even single molecules. Nathan Blow reports.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Pre-amplification in the context of high-throughput qPCR gene expression experiment
BMC Molecular Biology Open Access 11 March 2015
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
References
Sanchez, J.A., Pierce, K.E., Rice, J.E. & Wangh, L.J. Proc. Natl. Acad. Sci. USA 101, 1933–1938 (2004).
Raj, A., Peskin, C.S., Tranchina, D., Vargas, D.Y. & Tyagi, S. PLOS Biol. 4, e309 (2006).
Diehl, F. et al. Proc. Natl. Acad. Sci. USA 102, 16368–16373 (2005).
Kim, J.B. et al. Science 316, 1481–1484 (2007).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blow, N. PCR's next frontier. Nat Methods 4, 869–875 (2007). https://doi.org/10.1038/nmeth1007-869
Issue date:
DOI: https://doi.org/10.1038/nmeth1007-869
This article is cited by
-
Pre-amplification in the context of high-throughput qPCR gene expression experiment
BMC Molecular Biology (2015)
-
A nanoliter self-priming compartmentalization chip for point-of-care digital PCR analysis
Biomedical Microdevices (2015)
-
Rapid absolute determination platform of nucleic acid for point-of-care testing
Chemical Research in Chinese Universities (2015)
-
Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number
Analytical and Bioanalytical Chemistry (2009)