Abstract
TransAM™ Kits are highly sensitive enzyme-linked immunosorbent assay (ELISA)-based assays that facilitate the study of transcription-factor activation in mammalian tissue and cell extracts. They combine a fast, user-friendly format with a sensitive, specific assay. TransAM assays are up to 100 times more sensitive than gel-shift techniques and can be completed in less than five hours. The TransAM method eliminates the use of radioactivity and provides quantitative results. Moreover, the high-throughput stripwell format allows simultaneous screening of 1–96 samples.
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References
Renard, P. et al. Development of a sensitive multi-well colorimetric assay for active NFκB. Nucleic Acids Res. 29, E21 (2001).
Chen, Z. et al. Signal induced site-specific phosphorylation targets IκBα to the ubiquitin-proteasome pathway. Genes Dev. 9, 1586–1597 (1995).
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Challis, B., Heyman, J., Baer, S. et al. Simplified quantitation of activated transcription factors. Nat Methods 3, 658 (2006). https://doi.org/10.1038/nmeth907
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DOI: https://doi.org/10.1038/nmeth907
