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Optimizing knockdown of gene expression using the TriFECTa™ Dicer-substrate RNAi reagent system

Nature Methods volume 3, pages V–VII (2006)Cite this article

Abstract

Integrated DNA Technologies, Inc. (IDT) has developed a library of predesigned Dicer-substrate RNA duplexes. These potent RNA interference (RNAi) reagents are available in a kit (TriFECTa) that includes three specific RNA duplexes for target-gene knockdown plus three optimized control duplexes, which can be used to optimize transfection efficiency and other aspects of RNAi experiments.

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Figure 1: Transfection controls and dose optimization.
Figure 2: HPRT mRNA and protein knockdown.

References

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Authors and Affiliations

  1. Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, 52241, Iowa, USA

    Scott D Rose, Michael A Collingwood & Mark A Behlke

Authors
  1. Scott D Rose
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  2. Michael A Collingwood
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  3. Mark A Behlke
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Corresponding author

Correspondence to Mark A Behlke.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Rose, S., Collingwood, M. & Behlke, M. Optimizing knockdown of gene expression using the TriFECTa™ Dicer-substrate RNAi reagent system. Nat Methods 3, V–VII (2006). https://doi.org/10.1038/nmeth919

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