Supplementary Figure 4: Recordings from GCs across wakefulness state show state-dependent activity changes within individual GCs.

In a subpopulation of cells (n=6 cells from 6 animals), recordings were made from the same cell in both the awake and isoflurane anesthetized animal. (a) Spontaneous activity significantly increased in GCs across the anesthetized (black) to awake (grey) transition (U(5) = 25, p = 0.026). Mean firing rate ± s.e.m (red line and black lines respectively) are plotted. Anesthetized state mean ± s.d. = 2.86 ± 0.91; mean firing rate in the awake state = 13.00 ± 4.21. Paired data are plotted for each cell with the connecting line linking mean firing rate for an individual cell in each state. (b) Ranked tuning curves during 2 s odor presenta- tion and 1 s after odor offset are plotted for transitional cells in both the anesthetized (black line) and awake (grey line) state. (c) Tuning curve variance during both the 2 s odor presentation and 1 s following odor offset was greater in the awake state (during mean ± s.e.m = 17.04 ± 6.42; after = 26.94 ± 14.13) than in the anesthe- tized state (during = 1.64 ± 0.53; after = 3.53 ± 0.95, during U(5) = 22, p = 0.043; after U(5) = 25, p = 0.026). Individual data points represent one cell and mean ± s.e.m. are plotted as horizontal red and black lines respectively.