Supplementary Figure 5: Changes in breathing and GC firing with changes in locomotion.

(a) Example trace of GC activity (bottom), respiration (middle), and running velocity (top). Running was measured on an axially rotating Styrofoam ball via a rotary encoder. Locomotor activity was divided into 2 s bins, and running bins with RMS > 2 (red lines; Scale bar is 1 s and 4 cm/s for locomotion, 1 mV for breathing and 1.5 mV for GC spiking. (b) Breathing frequency increases with running. Breathing during the entire recording session was binned into 2 s intervals, and a mean rate during periods of rest or during periods of running was calculated. For all animals (n = 16 cells from 8 animals), breathing significantly increased during running (Mean breathing rate ± s.e.m. rest = 2.69 ± 0.08; running 3.68 ± 0.16; U(15) = 157, p = 0.000026). (c) GC activity is largely invariant with activity. Mean spike rate ± s.e.m. between periods of rest (8.15 ± 1.46 spikes/s) and periods of increased locomotion (8.08 ± 1.50 spikes/s) was not significantly different (U(32) = 1077, p = 0.71, left). Plotting individual data points (middle, left histogram, n = 33 cells from 19 animals) indicates a sub-population of cells is modulated with activity. Dotted red line runrate = stillrate.