Supplementary Figure 1: Physiology and anatomical recovery methods for GCs in awake mice.

(a) Mice were head-fixed on a Styrofoam ball by clamping a head bar affixed to the skull with dental cement. Mice could move freely in 1 dimension (forward or backward). An odor delivery tube (yellow) attached to an olfactometer was placed ∼ 5 cm from the animal's nose. Both odor and isoflurane (during anesthesia) were delivered through this tube. Water reward was delivered through a lick-port positioned below the odor delivery tube. (b) A typical recording from a GC across awake and anesthetized states. Upon several presentations of odor stimuli, animals were transitioned from awake to asleep using isoflurane anesthetic (red line). Where recording stability allowed, recordings were then conducted from the same cell in the anesthetized animal for several odor presentations. Then, anesthetic was turned off, and recording continued as the animal recovered. Timing of odor presentations are not shown. (c) Granule cells in the awake animal were labeled using the same methods as for granule cells in the anesthetized animal. Movement limited cell fill times, but anatomical location, cell size, and the presence of an EPL projecting apical dendrite (arrows) confirmed GC identity. Scale bar = 20 um. (GCL = granule cell layer, EPL = external plexiform layer).