Supplementary Figure 3: Granule cell layer neurons (putative GCs) and identified GCs do not differ in their properties.
From: Broadly tuned and respiration-independent inhibition in the olfactory bulb of awake mice
(a) Histogram of identified GCs (grey) and putative GCs (black) shows no difference in spontaneous activity between the two cell populations U(54) = 784, p = 0.8249. Identified GCs had a mean ± s.d. baseline rate of 7.30 ± 6.55 spikes/s (n = 28 cells from 19 animals) while putative GCs had a baseline rate of 7.99 ± 7.76 (n = 28 cells from 17 animals). (b) Ranked tuning curves for identified and putative GCs are similar. Identified GC curve is plotted in grey, putative GCs are plotted in black, and the combined curve is plotted in red. Only spike rates during the odor presentation are shown. (c) Response modulation index (RMI, see Online Methods) does not differ between the two groups. Each data point represents the RMI value for one cell. Mean ± s.d. RMI identified GC = 0.81 ± 0.42, n = 28 cells from 19 animals; RMI putative GC = 0.98 ± 0.54, n = 28 cells from 17 animals; U(54) = 706, p = 0.1890. (d) Coupling of spiking to breathing is not significantly different in the two groups (U(16) = 43, p = 0.2129). In both the identified GC (grey outline, n = 6 cells from 5 animals) and putative GC (black, n = 12 cells from 6 animals) groups, most cells show little coupling with breathing.
