Figure 6
From: STAT1 Negatively Regulates Spatial Memory Formation and Mediates the Memory-Impairing Effect of Aβ
Laminin β1 (LB1) mediates the memory-impairing effect of signal transducer and activator of transcription-1 (STAT1). Animals received Flag-vector+control siRNA, Flag-STAT1WT+control siRNA, or Flag-STAT1WT+LB1 siRNA co-transfections and were subject to (a) water maze learning and (b) probe trial test. (c) Gel pattern and analysis of STAT1 and LB1 protein expression after the probe trial test. Western blotting (WB) using anti-Flag antibody confirms the transfection and expression of Flag-STAT1WT. (d) Immunofluorescence showing the area of Cy3-LB1 siRNA transfection in the CA1 area. Cells that show both red fluorescence (Cy3) and blue fluorescence (DAPI) are the cells successfully transfected with the LB1 siRNA. Scale bar equals 200 μm for the left panel and 50 μm for the right panel. N=7 each group. Data are mean±SEM. *P<0.05, **P<0.01, and #P<0.001. #: compared with the control group and a: compared with Flag-STAT1WT+control siRNA group.
