Enhancing RNAi efficiency by inserting Nuclear factor-κB binding sequence into SiRNA expression cassette

Abstract

RNAi based applications have been widely used to manipulate cellular phenotypes, analyze gene function and as a promising molecular therapy, however, there are rare reports in how to target the SiRNA expression constructs from the cytoplasm to nucleus. In this study, we inserted NF-κB binding sequence into the SiRNA expression cassette to enhance RNAi efficiency, tested this propose by use of tRNAval and human H1 promoters, and eGFP and luciferase as reporter protein. The results showed that inserting NF-κB binding sequence at the 3’-terminal of the SiRNA expression construct could enhance RNAi efficiency.