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Lymphocyte migration

Restricted entry into lymph nodes

Nature Reviews Immunology volume 3, page 926 (2003)Cite this article

L-selectin mediates tethering and rolling of naive T cells in high endothelial venules (HEVs) prior to their migration to the PLNs. To investigate the importance of L-selectin shedding in this process, Ann Ager and colleagues generated endogenous L-selectin-deficient mice transgenic for either wild-type L-selectin (WT) or uncleavable L-selectin (L3P) in T cells. The percentage of T cells in the PLNs of these animals was not statistically different, providing preliminary evidence that naive T-cell trafficking is not regulated by L-selectin shedding.

The authors further investigated the importance of L-selectin shedding in T-cell rolling in vitro, using a hydrodynamic flow model, and T-cell trafficking in vivo after T-cell transfer to normal or recombinase-activating gene 1 (Rag1)-deficient mice. In vitro, the number of rolling L3P and WT T cells, as well as their rolling velocity and behaviour, was the same. In vivo, transferred WT and L3P naive T cells migrated in equal numbers to the PLNs. Together, these studies indicate clearly that naive T-cell homing to the PLNs is independent of L-selectin shedding.

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References

ORIGINAL RESEARCH PAPER

  1. Galkina, E. et al. L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes. J. Exp. Med. 198, 1323–1335 (2003).

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FURTHER READING

  1. Grabovsky, V., Dwir, O. & Alon, R. Endothelial chemokines destabilize L-selectin-mediated lymphocyte rolling without inducing selectin shedding. J. Biol. Chem. 277, 20640–20650 (2002).

    Article  CAS  Google Scholar 

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Authors
  1. Karen Honey
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Honey, K. Restricted entry into lymph nodes. Nat Rev Immunol 3, 926 (2003). https://doi.org/10.1038/nri1263

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  • DOI: https://doi.org/10.1038/nri1263

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