Supplementary Figure 5: HSP90 inhibition upregulates genes adjacent to ERVs that are repressed by KAP1-mediated H3K9me3 and SETDB1.
From: The evolutionary capacitor HSP90 buffers the regulatory effects of mammalian endogenous retroviruses
(a,b) A genome browser view of two loci showing an ERV and an adjacent gene, with ChIPseq and RNAseq profiles from mouse ES cells with indicated treatments and genotypes. The indicated coordinates correspond to mouse genome build mm10. At both loci, the ERV (IAPEz, green) is bound by KAP1 and shows the repressive mark H3K9me3 (brown box). The repressive mark is KAP1-dependent as the ChIPseq signal of H3K9me3 is reduced in KAP1 deletion. The adjacent gene is de-repressed in HSP90 inhibition, KAP1 deletion and for (b) also in SETDB1 deletion (RNAseq profiles in green and red).
