Figure 3
Proliferation of MEFs from WT and Jdp2KO mice. (a) Serum-starved cells (5 × 105) were cultured in DMEM plus 15% FCS for 18 h, stained with propidium iodide and subjected to FACS analysis to determine the percentage of cells in each phase of the cell cycle. (b) The percentage of MEFs in the S-phase was determined after re-stimulation in the 15% FCS at 4-h intervals, as in panel a. (c) MEFs were cultured in chamber slides in DMEM with 15% FCS for 12 h and then pulse-labeled with 10 μM BrdU for 3 h. Labeled cells were detected by immunostaining with BrdU-specific antibodies. Scale bars represent 100 μm. (d) Percentages of MEFs that were stained with BrdU-specific antibodies. Means of results of three experiments are shown. BrdU, bromodeoxyuridine; DMEM, Dulbecco's modified Eagle's medium; FACS, fluorescence-activated cell sorting; FCS, fetal calf serum; JDP2, Jun dimerization protein-2; KO, Jdp2-knockout; MEF, murine embryonic fibroblast; WT, wild type.
