Figure 6
JDP2 negatively regulates the expression of the gene for cyclin-A2. (a) Schematic representation of the promoter of the cyclin-A2 gene (nt −944 to +157) fused with a gene for the luciferase. The series of deletion mutants of cyclin-A2-luciferase are described in the text. The AP-1-like motif, AP-1 motif and CRE are indicated by a white box, a gray box and a black box, respectively. Point mutations in each motif are also indicated. (b) Reporter activities of cyclin-A2-reporter deletion constructs pA2-L, pA2-M and pA2-N. A 1-μg weight of cyclin-A2 promoter-luciferase reporter plasmid and 200 ng of pSV-luc were used for transfection of 5 × 104 Jdp2KO MEFs. Six hours after transfection, MEFs were washed, incubated for another 48 h and assayed. *P<0.001. (c) Luciferase activities of the point mutants of the cyclin-A2 promoter-luciferase constructs were determined as in panel b. (d) The pSV-luc plasmid was used as control for transfections in comparisons of Renilla luciferase activities of MEFs from Jdp2KO and WT mice. AP, activation protein; JDP2, Jun dimerization protein-2; KO, Jdp2-knockout; MEF, murine embryonic fibroblast.
