Figure 1
From: WW domain-mediated interaction with Wbp2 is important for the oncogenic property of TAZ
The WW domain of TAZ is important for its ability to confer anchorage-independent growth of MCF10A cells. (a) Soft agar growth assays were done using MCF10A cells transduced with expressing vector (control), Flag-TAZ, Flag-S89A and Flag-WWm. The colonies grown in soft agar after 1 month of culture were stained with thiazolyl blue tetrazolium bromide. (b) Lysates derived from the cells were analyzed by immunoblotting using anti-Flag antibody. (c) The colony number was quantified from three independent soft agar assays and presented as percentage relative to S89A, which is arbitrarily set as 100%. *P<0.05 versus TAZ. (d, e) The relative transcript levels of ITGB2 and CTGF in MCF10A cells stably expressing S89A, TAZ and WWm were assessed by real-time PCR. The relative expression level of ITGB2 and CTGF was presented as fold activation relative to vector control, which is set as 1. *P<0.05 versus TAZ.
