Figure 4
From: WW domain-mediated interaction with Wbp2 is important for the oncogenic property of TAZ
Suppression of Wbp2 expression inhibits, whereas overexpression of Wbp2 enhances TAZ-driven transformation of MCF10A cells. (a) The relative protein and transcript levels of Wbp2 were assessed by immunoblot and real-time PCR, respectively. The average level of Wbp2 transcript in vector-transduced cells is arbitrarily set at 1. *P<0.05 versus control. (b) S89A-driven anchorage-independent growth is suppressed by shRNA-mediated knockdown of Wbp2. S89A-expressing MCF10A cells (S89A) transduced with vector (control) or Wbp2 shRNAs (Wbp2-KD-1 or Wbp2-KD-2) targeting Wbp2 were grown in soft agar for a month and colonies were stained. (c) Quantification of soft agar colonies of S89A cells expressing Wbp2 shRNAs, the average from three independent experiments was presented. The average colony number from S89A cells expressing no shRNA targeting Wbp2 is arbitrarily set at 100%. *P<0.05 versus control. (d) Overexpression of Wbp2 enhances TAZ-driven transformation. MCF10A-vector and MCF10A-TAZ cells cotransduced with Wbp2 were used to perform soft agar assays. (e) Quantification of soft agar colonies from (d) and presented as percentage relative to TAZ+vector, which is arbitrarily set as 100%. *P<0.05 versus TAZ+control.
