Figure 5
From: WW domain-mediated interaction with Wbp2 is important for the oncogenic property of TAZ
Direct fusion of full-length Wbp2 and its C-terminal, but not N-terminal region, to WWm restore the transforming and gene-induction abilities of TAZ. (a) NIH-3T3 cells stably expressing the indicated constructs grown on soft agar are shown. (b) Lysates derived from cells expressing the indicated constructs were analyzed by immunoblotting using anti-Flag antibody (left) and anti-HA antibody (right). (c) Quantification of colony numbers for all the indicated constructs in comparison with vector-transduced cells. The average number derived from S89A-expressing cells is arbitrarily set at 100%. P<0.05 versus TAZ. (d) The relative expression levels of ITGB2 in MCF10A cells stably expressing the indicated constructs were assessed by real-time PCR. *P<0.05 versus TAZ.
