Figure 3
From: LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression
LARP1 regulate the stability of mTOR mRNA. (a) Western blot showing LARP1 knockdown with pooled siRNA. HSP60 was used as loading control. (b) Real-time PCR following transcription inhibition by actinomycin-D treatment in HeLa cells transfected with two pooled LARP1 siRNA or control siRNA. Fold changes relative to 18S, normalized to untreated control. (c) Luciferase production in HeLa cells transfected with firefly-luciferase reporter construct containing mTOR 3′UTR sequence normalized to the relative control plasmid (bottom panel: representation of the constructs used) in HeLa cells previously transfected with pooled siRNA to LARP1 or non-targeting siRNA. (d) Luciferase production in HeLa cells transfected with a renilla-luciferase reporter construct containing mTOR 5′UTR sequence normalized to the relative control plasmid (bottom panel: representation of constructs used) in HeLa cells previously transfected with pooled siRNA to LARP1 or non-targeting siRNA. Experiments were performed ⩾3 times. Data are mean±s.e.m. *P⩽0.05 and **P⩽0.001.
