Figure 2

KDM4B is expressed in hypoxic ovarian cancer tumors and EOC cell lines. (a) Representative images of TMA sections demonstrating association between elevated KDM4B staining with elevated CA-IX staining. IgG and hematoxylin and eosin (H&E) serve as negative and tissue structure controls, respectively. Scale bar, 50 μm. (b) Scatter plot of hypoxia (CA-IX staining) vs KDM4B staining in TMA sections. Blue diamonds, primary tumors (n=30); red squares, metastatic tumors (n=29). Statistical significance determined using Spearman’s correlation. P-value of <0.05 is considered significant. (c) Immunoblot detection of KDM4B and HIF-1α in a panel of ovarian cancer cell lines in normoxia (N, 21% oxygen) and in hypoxia (H, 0.5% oxygen). HIO-80 cells serve as nontransformed ovarian surface epithelium (OSE) control. HIF-1α indicates cellular hypoxia, β-actin serves as loading control.