Figure 6

KDM4B regulates peritoneal seeding and growth of ovarian cancer cells. (a) Representative ventral bioluminescent images of nude mice injected with SKOV3ip.1-luc-neo cells transduced with indicated shRNA constructs. Image represents the median tumor load for each group, 28 days after injection. (b) Longitudinal ventral bioluminescence measurement for all mice injected with SKOV3ip.1-luc-neo transduced with indicated shRNA. Data represents mean total radiance (photons/sec) for each group±s.e.m. (n=9 or 10 mice per group). Blue diamonds, shGFP; red squares, shK-1; and brown triangles, shK-2. (c) Dorsal bioluminescence for SKOV3ip.1 study, analyzed as in (b). (d) Representative images of mice injected with OVCAR8-luc-neo cells transduced with the indicated shRNA constructs. Image was captured at week 6 of study. (e) Ventral bioluminescence measurement for all mice injected with OVCAR8-luc-neo transduced with indicated shRNA constructs, analyzed as in (b). (f) Dorsal bioluminescence for OVCAR8 study, analyzed as in (b). Statistics for (b), (c), (e) and (f) were calculated with Kruskal–Wallis and Mann–Whitney U-tests (*P<0.05; **P<0.005). Blue asterisks, difference among all three groups; red asterisks, difference between shK-1 and shGFP; brown asterisks, difference between shK-2 and shGFP. (g) Box-and-whisker dot plot of total tumor weight for SKOV3ip.1 xenografts (n=7 per group). (h) Dot plot of tumor weight for OVCAR8 xenografts (n=9 or 10). (i) Dot plot of ascites fluid volume collected from OVCAR8 experiment (n=9 or 10 mice per group). For (g–i), statistical significance was determined using Mann–Whitney U-test relative shGFP control (*P<0.05). (j) Representative end point necropsy demonstrating differential tumor size and distribution in mice injected with SKOV3ip.1 or OVCAR8 cells. Scale bar, 1 cm. Visible tumors are circled in yellow. Note the large cluster of fused omental tumors in the shGFP controls as compared with the knockdown mice.