Figure 2
From: EphrinB1 promotes cancer cell migration and invasion through the interaction with RhoGDI1
Mapping the region of ephrinB1 for RhoGDI1 interaction by deletion mutagenesis. (a) Schematic representation of wt ephrinB1 or deletion mutants lacking 4, 20, 30, 34 or 60 amino acids from the C terminus or the extracellular domain (TmCyt). (b) HA-tagged wt or deletion mutants of ephrinB1 were co-transfected with Flag-tagged RhoGDI1 into HEK293T cells as indicated. Cell lysates were immunoprecipitated with HA antibody. Immunoprecipitates and total lysates were immunoblotted with HA or Flag antibodies. (c) Schematic representation of wt ephrinB1 or deletion mutants lacking amino acid 287–326, 327–334 or 335–342 of ephrinB1 C terminus. (d) HEK293T cells were co-transfected Flag-tagged RhoGDI1 along with wt or deletion mutants of ephrinB1 as indicated. Cell lysates were immunoprecipitated with HA antibody. Immunoprecipitates and total lysates were immunoblotted with HA or Flag antibodies.
