Figure 7

(A) Effect of COX-2 inhibition on v-FLIP/K13-induced colony formation and EndMT-linked genes. (a) Transformation assay schematic and recovery of transformed cells. (b) Untreated or COX-2 inhibitor-treated or solvent control-treated v-FLIP-HMVECs were plated in soft agar in the presence of the indicated doses of drugs or solvent controls at indicated times, and colonies were scored after 6 days. The values shown are mean±s.d. of two independent experiments performed in duplicate. Crystal violet staining of colonies formed from untreated, celecoxib or NS-398 solvent-treated and celecoxib or NS-398-treated v-FLIP-HMVECs. Light microscopy fields (original magnification, × 40) of untreated or COX-2 inhibitor-treated or solvent control-treated v-FLIP-HMVECs are shown. (c) MTT assay for the colonies solubilized from untreated, celecoxib or NS-398 solvent-treated and celecoxib or NS-398-treated v-FLIP-HMVECs. Histogram showing the MTT for the colonies shown in panels. The values shown are mean±s.d. of two independent experiments performed in duplicate. (B) Expression of (a) snail, (b) slug, (c) twist, (d) laminin-γ, (e) E-cadherin in the cells obtained from colonies derived in (A) ^* and ^**: statistically significant at P<0.01 and P<0.005, respectively.