Table 1 Sequences of real time primers used
From: COX-2/PGE2: molecular ambassadors of Kaposi's sarcoma-associated herpes virus oncoprotein-v-FLIP
Primer name | Sequence |
|---|---|
ICAM-1 | |
Forward | 5′-CATAGAGACCCCGTTGCCTAAA-3′ |
Reverse | 5′-TGGCTATCTTCTTGCACATTGC-3′ |
VCAM-1 | |
Forward | 5′-GGGAAGCCGATCACAGTCAA-3′ |
Reverse | 5′-ATGAGATGATCTCCTTTCAGTAAGTCTATC-3′ |
IL8 | |
Forward | 5′-GAAGGAACCATCTCACTGTGTGTAA-3′ |
Reverse | 5′-AAATCAGGAAGGCTGCCAAGA-3′ |
E-selectin | |
Forward | 5′-AATGTGTGGGTCTGGGTAGGAA-3′ |
Reverse | 5′-TCCACGCAGTCCTCATCTTTT-3′ |
Cyclophilin A | |
Forward | 5′-ATGGCACTGGTGGCAAGTCC-3′ |
Reverse | 5′-TTGCCATTCCTGGACCCAAA-3′ |
CCL2 | |
Forward | 5′-GATCTCAGTGCAGAGGCTCG-3′ |
Reverse | 5′-AAGCAATTTCCCCAAGTCTC-3′ |
CCL7 | |
Forward | 5′-GCACTTCTGTGTCTGCTGCT-3′ |
Reverse | 5′-TAGCTCTCCAGCCTCTGCTT-3′ |
CXCL2 | |
Forward | 5′-AACCGAAGTCATAGCCACAC-3′ |
Reverse | 5′-CAGGAACAGCCACCAATAAG-3′ |
CXCL10 | |
Forward | 5′-TGAGCCTACAGCAGAGGAA-3′ |
Reverse | 5′-TACTCCTTGAATGCCACTTAGA-3′ |
TGF-ß1 | |
Forward | 5′-GTCACCCGCGTGCTAATG-3′ |
Reverse | 5′-CACGTGCTGCTCCACTTTTA-3′ |
CXCL-2 | |
Forward | 5′-CGCCCAAACCGAAGTCATAG-3′ |
Reverse | 5′-AGACAAGCTTTCTGCCCATTCT-3′ |
CXCL-5 | |
Forward | 5′-TGGCCCCTTTCACAGAGTAG-3′ |
Reverse | 5′-CTAAAAACCCGACAGGCATC-3′ |
CXCL-3 | |
Forward | 5′-TCCCCCATGGTTCAGAAAATC-3′ |
Reverse | 5′-GGTGCTCCCCTTGTTCAGTATCT-3′ |
CXCL-6 | |
Forward | 5′-AGTTTACAGCTCAGCTAATGAAGTACTAAT-3′ |
Reverse | 5′-CGGTAAGACTTTAAGGAATGTATGATA-3′ |
IL-6 | |
Forward | 5′-CGGGAACGAAAGAGAAGCTCTA-3′ |
Reverse | 5′-GGCGCTTGTGGAGAAGGAG-3′ |
CCL-20 | |
Forward | 5′-CCAAGAGTTTGCTCCTGGCT-3′ |
Reverse | 5′-TGCTTGCTGCTTCTGATTCG-3′ |
MMP-2 | |
Forward | 5′-CAGGGAATGAGTACTGGGTCTATT-3′ |
Reverse | 5′-ACTCCAGTTAAAGGCAGCATCTAC-3′ |
MMP-9 | |
Forward | 5′-AATCTCTTCTAGAGACTGGGAAGGAG-3′ |
Reverse | 5′-AGCTGATTGACTAAAGTAGCTGGA-3′ |
MMP-10 | |
Forward | 5′-ACCTGGGCTTTATGGAGATATTC-3′ |
Reverse | 5′-TCATATGCAGCATCCAAATATGATG-3′ |
SLUG | |
Forward | 5′-CATGCCTGTCATACCACAAC-3′ |
Reverse | 5′-GGTGTCAGATGGAGGAGGG-3′ |
SNAIL | |
Forward | 5′-GAGGCGGTGGCAGACTAG-3′ |
Reverse | 5′-GACACATCGGTCAGACCAG-3′ |
TWIST | |
Forward | 5′-CGGGAGTCCGCAGTCTTA-3′ |
Reverse | 5′-TGAATCTTGCTCAGCTTGTC-3′ |
E-Cadherin | |
Forward | 5′-CAGAAAGTTTTCCACCAAAG-3′ |
Reverse | 5′-ACTGAACCTGACCGTACA-3′ |
Laminin-γ1 | |
Forward | 5′-CTCCATCAACCTCACGCTG-3′ |
Reverse | 5′-CGG CTGGTGTGGAACTTG-3′ |
GAPDH | |
Forward | 5′-GTTCGACAGTCAGCCGCATC-3′ |
Reverse | 5′-GGAATTTGCCATGGGTGGA-3′ |
HPRT | |
Forward | 5′-GGACAGGACTGAACGTCTTGC-3′ |
Reverse | 5′-CTTGAGCACACAGAGGGCTACA-3′ |
Tubulin | |
Forward | 5′-GTACCGTGGTGACGTGGTTC-3′ |
Reverse | 5′-CTTGGCATACATCAGGTCAA-3′ |
