Figure 5
From: The EGFR family members sustain the neoplastic phenotype of ALK+ lung adenocarcinoma via EGR1
EGR1 is regulated by ALK and EGFR, and promotes survival of H2228 cells. (a) H2228 and H3122 cells were treated with CEP-28122 and/or gefitinib (G) in 0.05% FCS for 24–48 h and 6–12 h, respectively. Total RNA was extracted and analyzed for EGR1 expression by real-time PCR. Significance according to the t-test corresponded to *P<0.05 and **P<0.005. (b, c) H2228 (mock) and H2228 transduced with EGR1 cDNA (pWPI-EGR1) were treated with CEP-28122 and G, in single or in combination in 0.05% FCS for 96 h and 120 h, respectively. Cell viability was evaluated by Cell Titer-Glo Luminescent kit (b) and the percentage of apoptotic cells was measured by tetrametylrodamine methyl ester staining followed by fluorescence-activated cell sorting analysis (c). Significances * and ** are referred to the corresponding untransduced cells (mock). Results are representative of three different experiments. (d) Cells treated with CEP-28122 and/or G in 0.05% FCS for 24 h were collected for western blot analysis with the indicated antibodies.
