Figure 2

Interaction analysis of NoLS sequences. Peptides corresponding to the putative NoLS were dansylated at their N terminus and titrated with NPM1 constructs. The static fluorescence spectra are shown in insets, while the experimental maxima and their fit according to equation 1 (see Materials and Methods) are reported as a function of NPM1 concentrations in the main panels as follows: (a) Interaction between Fbw7γ* and Nter-NPM1. (b) Interaction between Fbw7γ* and Cter-NPM1. (c) Interaction between CENP-W* and Nter-NPM1. (d) Interaction between Tat* and Nter-NPM1. Peptides sequences are reported in Table 1.