Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
T. J. Povsic, S. A. Strobel, and P. B. Dervan, J. Am. Chem. Soc., 114, 5934 (1992) and references therein.
Only a supercoiled plasmid DNA, which is allegedly activated for the cleavage by intramolecular strain, was cleaved partially via hydrolytic pathway by use of the combination of a ruthenium complex and divalent transition metal ion: L. A. Basile, A. L. Raphael, and J. K. Barton, J. Am. Chem. Soc., 109, 7550 (1987).
J. Chin, M. Banaszczyk, V. Jubian, and X. Zou, J. Am. Chem. Soc., 111, 186 (1989).
DNA is far more (around 105 fold) resistant to alkaline hydrolysis than RNA, since the intramolecular reaction by the 2′-OH residue is not available.
Y. Matsumoto and M. Komiyama, Chem. Express, 7, 785 (1992).
The structure of the double-stranded portion formed in the duplex of the 35-mer DNA has been precisely determined by 1H-NMR spectroscopy: S.-G. Kim, L.-J. Lin, and B. R. Reid, Biochemistry, 31, 3564 (1992).
The ratio of Tp to pT, accumulated in the reaction mixture, was around 1.5. Note that the ratio is governed by the rate constants for both the formation of them and their hydrolysis and thus is not directly associated with the ratio of the P-O(5′) scission and the P-O(3′) scission in the hydrolysis of the phosphodiester linkage in DNA. The reversed-phase HPLC also showed formation of quite small amounts of pTp and TpTp. In the ion-exchange HPLC in Figure 1, all the monomers and the dimers eluted out in the peak of 0–1 min.
D. S. Sigman, Acc. Chem. Res., 19, 180 (1986).
P. B. Dervan, Science (Washington D.C.), 232, 464 (1986).
This finding is consistent with the previous report that La(III) salt is active for the hydrolyses of mono-phosphates of ribonucleosides: M. Shimomura and F. Egami, Bull. Chem. Soc. Jpn., 26, 265 (1953). However, the proposal by the authors that La(III) is totally inactive for the DNA hydrolysis is incorrect (see the text).
R. M. Milburn, M. Gautam- Basak, R. Tribolet, and H. Sigel, J. Am. Chem. Soc., 107, 3315 (1985).
J. Ciesiolka, T. Marciniec, and W. Krzyzosiak, Eur. J. Biochem., 182, 445 (1989).
R. W. Hay and N. Govan, J. Chem. Soc., Chem. Commun., 714 (1990), and references therein.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Komiyama, M., Matsumoto, Y., Hayashi, N. et al. Hydrolysis of OligoDNAs by Lanthanide Metal(III) Chloride. Polym J 25, 1211–1214 (1993). https://doi.org/10.1295/polymj.25.1211
Issue date:
DOI: https://doi.org/10.1295/polymj.25.1211
Keywords
This article is cited by
-
Solubilization of lanthanide ions by cyclodextrins in basic aqueous solutions
Journal of Inclusion Phenomena and Molecular Recognition in Chemistry (1994)
