Abstract
Formation of ion complexes between poly(amido amine) (PAMAM) dendrimer HCl salt (1) (ethylenediamine core, generation G = 3.0 and 5.0) and poly(L-glutamic acid) sodium salt (2) (Mw, 1.3×104, 1.5×104, and 8.9×104) in water and phosphate buffers (pH 6.0, 6.9, and 7.9) was investigated by pH, turbidity, and viscosity measurements and electrophoresis analysis. In pH and turbidity measurements in water, the highest turbidity and neutralization were observed around a 1 : 1 charge ratio, demonstrating that not only primary amines on the surface but also internal tertiary amines of 1 participate in the ion complex formation. When lower molecular weight 2 (Mw, 1.3×104 and 1.5×104) were used for complexation in water and phosphate buffers, mixtures of 1 and 2 did not become turbid except around the point of neutralization in water. In electrophoresis, the non-turbid mixtures of 1 (G = 5.0) and 2 (Mw, 1.5×104) showed a characteristic broad band, meaning ion complex formation. Viscosity of the aqueous solutions of a mixture of 1 and 2 (Mw, 1.5×104) was lower than that of 2 (Mw, 1.5×104). The conformation of 2 in the complex was proved to be a random coil by circular dichroism (CD) analysis. These results were interpreted in terms of water-soluble ion complex formation due to the globular shape and molecular structure of 1.
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Tsutsumiuchi, K., Aoi, K. & Okada, M. Ion Complex Formation between Poly(amido amine) Dendrimer HCl Salt and Poly(L-glutamic acid) Sodium Salt. Polym J 32, 107–112 (2000). https://doi.org/10.1295/polymj.32.107
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DOI: https://doi.org/10.1295/polymj.32.107
