THE UPTAKE OF ¹²⁵I - LABELLED RAT ALLOANTIBODY AND ITS LOSS AFTER COMBINATION WITH ANTIGEN

Abstract

¹²⁵1 - labelled transplantation alloantibody was injected into long surviving, enhanced and recently transplanted, non-enhanced kidney grafts. Both types of grafts bound the same amount of specific antibody and subsequently released it at a similar rate indicating a dynamic state of antigen turnover by renal allografts and an undiminished number of free antigen sites in long surviving enhanced grafts. In vitro studies showed that ¹²⁵1 - labelled alloantibody which combined specifically with target cells exhibited similar phenomenon as grafts in vivo. After 15-18 hours of incubation, 27.5-55.8% of bound label was shed from target cells into surrounding medium and serial elution studies showed that after 183 hours of incubation in vitro > 90% of counts specifically attached were shed. The released labelled material contained little specific reactivity indicating that either released antibody was aggregated or degraded, the radiolabel had been stripped from the antibody molecule or the antibody had been released combined with antigen i.e. complex. Subsequent studies utilizing gel filtration and radioimmunoassay were employed in attempts to characterize released material.

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