Abstract
Rabbit antiaera to autologous T and B lymphoblast cell lines HSB-2 and SB, after reciprocal absorption, were found to detect human T and B lymphocyte antigens (HTLA and HBLA antigens). When tested by indirect immunofluorescence and C' dependent cytotoxicity, the anti-T cell line serum reacted with T cells but not B cells whereas the anti-B cell line serum reacted with B cells but not T cells. These two antisera also reacted with peripheral blood null cells prepared by nylon column filtration and E rosette depletion. The sum of the percentages of null cells reacting with each antiserum approximated 100%. Null cell suspensions depleted of cells reacting with one antiserum showed a reciprocal increase in cells reacting with the opposite antiserum.
These findings indicate that most, if not all, null cells express either HTLA or HBLA antigens. It appears therefore that some null cells are related to the T cell line of differentiation whereas others are related to the B cell line of differentiation. (Supported by USPHS NIH Grant CA 17534 and Contract l-CP-33333. J. Kaplan is recipient of NIH Research Career Development Award CA 00188).
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Kaplan, J., Peterson, W. DETECTION OF T AND B LYMPHOCYTE ANTIGENS ON TWO MAJOR NULL CELL SUBSETS. Pediatr Res 11, 489 (1977). https://doi.org/10.1203/00006450-197704000-00713
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DOI: https://doi.org/10.1203/00006450-197704000-00713
