Abstract
CBL “null” cells prepared by nylon column filtration and E rosette depletion constitute roughly 10% of the total Ficoll-Hypaque separated mononuclear cells. This subset, although heterogeneous, is constituted mainly of cells (80%) which possess human T lymphocyte differentiation antigens and/or receptors for the Fc portion of IgG (45% to 58%). Following incubation of CBL*with thymosin (Fraction V), the following changes were seen: E RFC increased from 9% ± 10 to 28% ± 15 (p < .05); Tγ increased from 58.5% ± 14 to 66. 8% ± 12 (p < .05). Employing a double rosette assay using a chicken erythrocyte-IgG complex (CEA) developed in our laboratory, instead of sheep erythrocyte-IgG complex (sheep EA), there was an increase only in the Tγ precursors from 45% ± 7 to 88% ± 5.6 (p < .02), but not in E RFC nor in double rosettes. Since 5 adult peripheral blood specimens tested showed an average of 5.5% double rosettes, we doubt that steric hindrance is the cause of this observation. Rather, the Tγ precursor appears to be more avid for CEA than the mature Tγ cell, or Fc receptors induced by thymosin appear before E receptors. This subset could be a regulator of maternal-fetal immune responses and play an important role in plasma cell differentiation in the fetus.
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Bernales, V., Bellanti, J. 683 E-ROSETTE-FORMING CELLS (E RFC) AND Tγ CELLS (SUPPRESSOR T) PRECURSORS IN CORD BLOOD LYMPHOCYTES (CBL). RESPONSIVENESS TO THYMOSIN FRACTION. Pediatr Res 12 (Suppl 4), 477 (1978). https://doi.org/10.1203/00006450-197804001-00688
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DOI: https://doi.org/10.1203/00006450-197804001-00688
