Abstract
A pediatric patient (pt) with Graves disease and circulating anti-Tg antibody (ab) levels developed ICGN characterized by mesangial, subendothelial and subepithelial dense deposits by electron microscopy. Renal cortex tissue examined by direct immunofluorescence (IF) demonstrated granular glomerular immunoglobulin (Ig) and complement (C1) deposition. Indirect IF demonstrated granular glomerular and mesangial deposits of HuTg. Indirect IF also showed that eluates of the pt's kidney contained (ab) reactive with thyroid follicular cells and colloid. CICs were demonstrated in the pt's sera by both the Raji cell assay and CIq solid phase assay. The indirect IF Raji assay demonstrated HuTg in ICs bound to the Raji cells. That HuTg was the antigenic component of CICs was further documented by a marked decrease Raji cell binding of CICs in the pt's sera when the sera was preincubated with HuTg. ICs eluted from both renal cortex tissue and CICs bound to Raji cells were analyzed by immunoelectrophoresis techniques and found to contain HuTg. We concluded that HuTg-anti-HuTg CICs have nephritogenic capacity and are important in induction and perpetuation of ICGN.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jordan, S., Buckingham, B., Olson, D. et al. DEMONSTRATION OF CIRCULATING IMMUNE COMPLEXES (CICs) CONTAINING HUMAN THYROGLOBULIN (HuTg) IN A PATIENT WITH IMMUNE COMPLEX GLOMERULONEPHRITIS (ICGN) MEDIATED BY THYROGLOBULIN ANTI-THYROGLOBULIN IMMUNE COMPLEXES. Pediatr Res 14, 1013 (1980). https://doi.org/10.1203/00006450-198008000-00237
Issue date:
DOI: https://doi.org/10.1203/00006450-198008000-00237
