Abstract
A RRA for hGH has been developed using the 100,000 g pellet from human liver tissue homogenate obtained from a donor of kidney transplantation. This assay is sensitive in the physiological range of hGH (1.9 - 30.0 ng/ml). Human prolactin and human placental lactogen did not compete with hGH on its receptor. Dilution curves parallel to the standard curve were obtained by receptor-active hGH found in the plasma of 7 LTD patients, four stimulated hGH healthy children and 2 acromegalics. Examples of results as hGH ng/ml are:
Our findings using this highly specific RRA for hGH suggest that circulating hGH in LTD is biologically active and that the primary defect lies either in the receptors or in the intra-cellular mechanism generating somatomedins.
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Eshet, R., Peleg, S., Josefsberg, Z. et al. Characterization of hGH from patients with LTD by a human liver radio-receptor assay (RRA). Pediatr Res 15, 89 (1981). https://doi.org/10.1203/00006450-198101000-00109
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DOI: https://doi.org/10.1203/00006450-198101000-00109
