946 ANTIBODY DEPENDENT CELL MEDIATED CYTOTOXICITY (ADCC) IN SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) AND JUVENILE DERMATOMYOSITIS (JDMS) AND THE EFFECT OF IN VITRO PREDNISOLONE

Abstract

Children with clinically inactive SLE and JDMS may have abnormal immune function reflecting underlying defects. ADCC, mediated by Fc receptor bearing cells, may play a role in these diseases by eliminating autoantibody coated cells; it is abnormal in some adult SLE patients. ADCC and its inhibition by prednisolone have not been studied in children with SLE or JDMS. We have shown that ADCC is inhibited by in vitro prednisolone in healthy adults; the purpose of this study is to determine if ADCC mediated by peripheral mononuclear cells from children with SLE and JDMS is inhibited by prednisolone. Chang cells labelled with 51-chromium were used as targets. Neither 100 nor 50 ug/dl prednisolone significantly decreased ADCC (72.0±18.8 at 64:1 effector: target (E:T) ratio). 6 of these children had clinically inactive disease. 6 SLE patients studied showed a correlation of ADCC with total hemolytic complement (r=.62). There was no significant decrease in ADCC when prednisolone was added to cells from 4 children with DMS (70.3±14.3 at 64:1 E:T), only 1 of whom had active disease. It is concluded that in vitro prednisolone inhibits ADCC from normal adults but not 7 of 9 children with SLE or 4 children with DMS. It is speculated that lack of ADCC response to in vitro prednisolone may reflect altered cell subpopulations in children with inactive and active SLE and JDMS.

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