Abstract
The L/S ratio test associated with the determination of phospha-tidylglycerol (PG) allows to obtain the lung profile of the fetal pulmonary maturity. In this study the L/S ratio is compared with the measurement of PG in human amniotic fluid. Both determinations were done in the same sample at the same time. The percentage of PG was determined in the amniotic fluid acetone precipitated fraction by adding the total density readings of the phospholipid compounds and calculating the percentage of PG when present. This procedure was performed in 1,354 amniotic fluid samples obtained by transabdominal amniocentesis. It was found that the higher the L/S ratio the greater the amount of PG present in the amniotic fluid. (see Table)
PG was detectable in 90% of the samples having an L/S ratio of 3 or more. Therefore the presence of PG with 10% of error, can be predicted when L/S ratio is greater than 3, thus avoiding identification of the compound by two-dimensional chromatography, since performance of this procedure may be more complicated according to available facilities.
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Bustos, R., Henriquez, R., Kulovich, H. et al. 21 L/S RATIO AND PHOSPHATIDYLGLYCEROL IN AMNIOTIC FLUID. Pediatr Res 15, 179 (1981). https://doi.org/10.1203/00006450-198102000-00041
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DOI: https://doi.org/10.1203/00006450-198102000-00041
