Radioimmunoassays for hFSH and hLH: Using an Immunoadsorbent Separation Technique and Human Serum as Standard Matrix

Abstract

Radioimmunoassays for the determination of the gonadotropins hLH and hFSH employing standards based on a human serum matrix were developed. The serum was depleted of the glycoproteohormones (hLH, hFSH, hCG, hTSH) by an immunadsorption technique.

The binding of the immuncomplex (AgAb) and the phase separation was accomplished by a novel PEG accelerated double antibody solid phase technique.

The requirement of identity between standard and unknown serum sample was fulfilled by using serum matrix for standards. As a consequence identical reaction kinetics were obtained and systematic errors avoided. Especially in pediatric diagnoses the fluctuation of the plasma protein content does not influence the separation by the immunoadsorption method. The methodological characteristics of the assays permit a reliable determination of hLH and hFSH values, especially in the low concentration range, were the normal values of children are encountered.

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