257 CEREBRAL METABOLISM IN THE PUP OF A CANINE DIABETIC MOTHER (IDM)

Abstract

Term pups from 6 insulin diabetic dogs were compared to controls from 6 litters after 0,3,6,9 and 24 hrs of neonatal fasting. Fetal cerebral glucose (CG) levels were elevated in IDMs (N=8) (5.45±0.98 vs 1.87±0.28 μ mol/g)⁺ but equalized by 3 hrs as IDM levels declined and controls increased. Cerebral glycogen(Gly) was increased in fetal IDMs (3.45±0.61 vs 1.66±0.16). Gly was equivalent to controls at 3,6 and 9 hrs and was lower than controls at 24 hrs (1.54±0.21 vs 2.46±0.24). Cerebral glucose-6-phosphate, fructose-6-phosphate, phosphoenolpyruvate, pyruvate and lactate (L) were similar during the day except L was elevated in IDMs at 24 hrs. Cytoplasmic NAD/NADH ratios were unaffected, as were citrate, α-ketoglutarate and malate. IDM calculated oxaloacetate levels were elevated at 0,3 and 9 hrs. ATP, ADP, AMP and phosphocreatine levels were equivalent at all times. Cerebral glutamate and aspartate were higher among IDMs throughout the study while alanine was elevated only after birth. Glutamine was higher in the IDMs at 6 and 24 hrs. Cerebral ammonia levels were not affected. Though gamma aminobutyrate was elevated in fetal IDMs (1.26±0.14 vs 0.915±0.15) this did not persist. These data suggest that maternal canine diabetes 1) ↑fetal cerebral Gly stores, and 2) ↑fetal and neonatal cerebral amino acid pools. As CG is an important precursor of cerebral amino acids, ↑fetal CG provision may result in augmented cerebral amino acid pools. Alternately, ample G supply may spare cerebral amino acid utilization. Nonetheless, fetal cerebral substrate stores are enhanced.

^*Deceased ⁺Mean^±SE

Log in or create a free account to read this content

Gain free access to this article, as well as selected content from this journal and more on nature.com

Access through your institution

or

Sign in or create an account

Continue with Google

Continue with ORCiD