Abstract
Previously we reported that PB administration during late pregnancy caused defects in sexual maturation of female offspring (delayed vaginal opening, irregular cycle and infertility). To elucidate the mechanism of these effects, inhibitors of protein RNA and DNA synthesis were studied. Time pregnant rats (S-D,CD strain) 5 per group, were injected, SC with different agents from 17-20 days of gestation.
Results show that cycloheximide (CY), a protein synthesis inhibitor, and hydroxyurea (OH-u), a DNA synthesis inhibitor, when combined with PB significantly reduced reproductive dysfunction. However, α-amantinin (α-Am), an RNA synthesis inhibitor, failed to protect the offspring from PB induced malformations. No inhibitor alone had any effect on reproductive function. Results suggest that prenatal PB produces its effect via new protein synthesis.
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Gupta, C., Yaffe, S. 333 REVERSAL OF PHENOBARBITAL (PB) INDUCED ALTERATION IN SEXUAL DIFFERENTIATION BY CYCLOHEXIMIDE AND HYDROXY UREA. Pediatr Res 15 (Suppl 4), 495 (1981). https://doi.org/10.1203/00006450-198104001-00344
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DOI: https://doi.org/10.1203/00006450-198104001-00344
