Abstract
NK activity was studied in cord blood lymphocytes using K562 target cells. The mononuclear layer was depleted using an adherence technique. High effector to target ratios (50:1 and 100:1) were used in an 18 hour Chromium(51) release assay. K562 lysis was then measured after cord blood lymphocytes were incubated with interferon (1000 U/ML and 2000 U/ML).
NK activity was found to be statistically lower than adult means with both E:T ratios at baseline activity and after interferon enhancement. However, at the 100:1 ratio cord blood lymphocytes enhanced with 2000 U/ML of interferon showed a decline in the enhancement curve which could represent the effect of higher concentrations of interferon on suppressor lymphocyte subpopulations in the neonate.
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Mohandes, A., Brudno, D. NATURAL KILLER (NK) LYMPHOCYTE ACTIVITY ENHANCED BY INTERFERON. Pediatr Res 18 (Suppl 4), 261 (1984). https://doi.org/10.1203/00006450-198404001-01008
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DOI: https://doi.org/10.1203/00006450-198404001-01008
