Abstract
Last year, Tannous (Ped Research #1183) reported plasma chemotactic inhibitors in hemophiliacs receiving Factor VIII concentrates. To confirm this finding,and to assess total granulocyte function we tested 11 severe hemophiliacs 21-64 years old (median 25) (8 on Factor VIII and 3 on cryo). All had normal T and B cell numbers but significantly decreased T4/T8 ratios 0.8±.6 cryo, p <.01 FVIII 0.8±.2 p <.001 vs control 1.5±.3. Cells were normal when tested for bacteriacidal killing against S.aureus, FMLP (N-Formyl-L-Methiony-L-Leucyl-L-Phenylalanine) stimulated aggregation and cyto B/FMLP stimulated superoxide production.
Human PMNs were tested for chemotactic activity using the Gallin modification of the Boyden Chamber technique with prelabelled Cr51 PMNs stimulated by E.Coli Endotoxin with and without patient serum. In contrast to previous findings, no abnormality was noted.
In addition further initial studies show no change in chemotactic activity when normal donor cells were incubated with commerical Factor VIII preparations. Thus in hemophiliacs with abnormal cellular immunity we were unable to demonstrate any cellular or serum defects in chemotaxis or in other granulocyte functions. A larger series of patients will be studied to confirm this.
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Kempert, P., Katz, J., Mallett, C. et al. NORMAL CHEMOTAXIS AND OTHER GRANULOCYTE FUNCTIONS IN HEMOPHILIACS WITH ABNORMAL T HELPER/T SUPPRESSOR CELL RATIOS. Pediatr Res 18 (Suppl 4), 243 (1984). https://doi.org/10.1203/00006450-198404001-00899
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DOI: https://doi.org/10.1203/00006450-198404001-00899
