Abstract
Previous studies by others have demonstrated preferential loss of the high molecular weight vWf multimers after infusion of purified 125I-radiolabeled vWf but this abnormality might be the result of purification and radiolabeling the vWf. We studied plasma factor VIII molecular complex serially in a patient with severe vWd after transfusion with cryoprecipitate (cryo) and determined the multimeric composition of vWf by SDS-agarose electrophoresis. Studies before and after infusion of 1 donor unit cryo per 5 kg body weight were as follows:
Multimeric analysis of vWf showed progressive loss of the larger multimers. A vW antigen fragment was identified following subsequent infusions. Thus, with infusion of vWf from cryo, we have confirmed the preferential loss of large multimers. The vW antigen frag may be the result of in vivo or in vitro proteolysis.
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Gill, J., Endres-Brooks, J. & Montgomery, R. 907 ALTERATION OF TRANSFUSED VON WILLEBRAND FACTOR (VWF) IN A PATIENT WITH SEVERE VON WILLEBRAND DISEASE (VWD). Pediatr Res 19, 262 (1985). https://doi.org/10.1203/00006450-198504000-00937
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DOI: https://doi.org/10.1203/00006450-198504000-00937
