Abstract
The effect of C. Parvum induced RES activation on la and Fc expression of hepatic nonparenchymal cells (NPC) and alveolar macrophage (AM) subpopulations was studied by flow microfluoremetry (FMF). DBA/2J mice were injected I.V. with 1.4 mg C.Parvum. After 14 days, NPC were isolated by collagenase perfusion of the portal vein followed by separation on Metrizamide and Ficoll-Hypaque gradients. AM from the same animals were then isolated by tracheal lavage. Fc expression was determined by incubating the cells with saturating concentration of rabbit IgG dimaers followed by FITC labelled goat anti-RIg. la expression was determined by H2-specific FITC or biotinylated anti-la and Texas Red Avidin. Percentage of positive cells is shown on following table:
The intensity of la expression, as determined by median fluorescence intensity (FMI), increased over 20% in both AM and NPC after C.Parvum. Although Fc expression increased in AM, the fluorescence histogram for Fc expression of Fc+Ia+ NPC became variable with an overal decreased FMI in the C.Parvum treated mice. These data suggest a differential response to C.Parvum stimulation in number of Fc surface receptors expressed among RES subpopulations
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Magilavy, D., Hundley, T. & Katona, I. 1004 RETICULOENDOTHELIAL ACTIVATION, DIVERSITY IN MACRO-PHAGE SUBPOPULATIONS. Pediatr Res 19, 278 (1985). https://doi.org/10.1203/00006450-198504000-01034
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DOI: https://doi.org/10.1203/00006450-198504000-01034
