Abstract
Human leukemic lymphoblastic cell lines (Molt-4, Raji) were treated for 24 hrs with 0.2 μM Methotrexate (MTX). After 12 hrs, intracellular dCTP and dTTP concentrations decreased to 20% of the values of untreated cells. We noted a significant increase of UMP, UTP and CTP concentrations under these conditions. At the same time dATP and dGTP levels dropped to 30% and 50%, respectively. Accumulation of cells in G1 phase and decrease of cells in G2+M phase (to almost zero) was observed, after treatment with 0.2 μMMTX. The effect of 0.02 μM MTX on intracellular purine and pyrimidine concentrations was less pronounced, than that of 0.2 μM MTX, except for dCTP. In contrast to a decrease of dCTP, observed in experiments with 0.2 μM MTX, an increase of dCTP was measured in experiments with 0.02 μM MTX. Cell cycle phase distribution studies with 0.02 μM MTX, revealed an accumulation of cells in early S phase and decrease of cells in G1 phase and G2+M phase.
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De Abreu, R., Bökkerink, J., Bakker, M. et al. INFLUENCE OF METHOTREXATE ON PURINE AND PYRIMIDINI POOLS AND ON CELL PHASE DISTRIBUTION OF CULTURED HUMAN LYMPHOBLASTS: 50. Pediatr Res 19, 752 (1985). https://doi.org/10.1203/00006450-198507000-00070
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DOI: https://doi.org/10.1203/00006450-198507000-00070
