Abstract
2',5'-oligoadenylate(2-5A) synthetase is an interferon(IFN)-induced enzyme, which has been considered to play a central role in antiviral activity. In general, the increased susceptibility to viral infections are common in severe combined immunodeficiency. Therefore, using human T lymphoblastoid cell line treated with deoxycoformycin as an in vitro model of adenosine deaminase deficiency, we investigated the effect of deoxyadenosine(dAdo) on the 2-5A synthetase activity induced by IFN. To measure 2-5A synthetase activity, we used two different assay systems, Sokawa's and Baglioni's methods. In the former, the net 2-5A synthetase activity was determined after semipurification of the enzyme through poly(I):poly(C) agarose gel, whereas in the latter method, the enzyme activity in the crude extract was detected. The enzyme activity was constant regardless of changes of dAdo concentration by Sokawa's method. However, in Baglioni's method, the activity decreased as the dAdo concentration increased. The activity of 2'-phosphodiesterase in the crude extract, which is also induced by IFN and degrades 2-5A, was similar in spite of changes of dAdo concentration. Inhibitor of 2-5A synthetase which clearly differs from dATP(a competitive inhibitor of the enzyme) accumulated in dAdo-treated cells could be induced in our system.
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Heike, T., Katamura, K., Kubota, M. et al. Inhibitor of 2' ,5′-oligoadenylate synthetase induced in human T lymphoblastoid cell line treated with deoxyadenosine, deoxycoformycin and interferon: 83. Pediatr Res 19, 757 (1985). https://doi.org/10.1203/00006450-198507000-00103
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DOI: https://doi.org/10.1203/00006450-198507000-00103
