Abstract
The resistance of cytotoxic T cells (Tc) to CSA may be related to the way they handle purines such as Ado. Ado has previously been shown to be toxic to Tc. We tested the effect of CSA, Ado, and the ADA inhibitor EHNA on the cytotoxicity of IL-2 dependent cloned Tc cells derived from C57/BL6 mice directed against syngeneic targets bearing SV40 antigen. No effect was seen if Tc were treated only with Ado 10−3M, EHNA 10−3M or CSA 10 ug/ml or 25 ug/ml.
Significant inhibition of cytotoxicity occurred when Ado, EHNA, and CSA (25 ug/ml) were combined. Mild inhibition of cytotoxicity occurred when Ado was combined with EHNA. Marked inhibition occurred if Ado was added. These data suggest that if ADA is inhibited, CSA can potentiate the toxicity of Ado to cytototoxic T cell function. This may have relevance to antirejection therapy in organ transplantation.
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Lum, C., Jennings, S., Wanner, F. et al. INHIBITION OF T CELL CYTOTOXICITY BY CYCLOSPORINE (CSA) ADENOSINE (ADO), AND AN INHIBITOR OF ADENOSINE DEAMINASE (ADA): 113. Pediatr Res 19, 762 (1985). https://doi.org/10.1203/00006450-198507000-00133
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DOI: https://doi.org/10.1203/00006450-198507000-00133
