Abstract
The uptake of (8-14C)hypoxanthine by human erythrocytes, suspended in a Tris glucose NaCl isotonic medium, has been studied at 37°C in pH 6.7-8.0 range. In the presence of inorganic phosphate (3-10 mM), hypoxanthine was taken up by the cells and incorporated into IMP. The rate of hypoxanthine uptake was markedly stimulated by decreasing the pH and/or the oxygen partial tension in the incubation medium. The effect exerted by oxygen was more evident in the acidic pH range and could be overcome by removing the gas. At pH higher than 7.5, hypoxanthine was not appreciably taken up by the cells even in anaerobic conditions. Carbon monoxide inhibited hypoxanthine uptake as well as pure oxygen atmosphere.
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Salerno, C., Gerber, G. & Giacomello, A. REGULATORY ASPECTS OF HYPOXANTHINE UPTAKE BY HUMAN ERYTHROCYTES: 178. Pediatr Res 19, 773 (1985). https://doi.org/10.1203/00006450-198507000-00198
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DOI: https://doi.org/10.1203/00006450-198507000-00198
