Abstract
The respiratory distress syndrome of preterm infants can be effectively treated by endotracheal administration of the bovine surfactant preparation SF-RI 1. Though the protein content of SF-RI 1 is less than 1 96, immunological reactions of the human infant against the bovine protein antigens have to be considered. Antigenicity of SF-RI 1 was demonstrated by antibody production in rats and rabbits.
Methods: An ELISA with a lowest detection limit of 1 ng/ml specific antibody against bovine surfactant was set up. Sera of 52 preterm infants were tested before as well as 2, k and 6 weeks after the administration of SF-RI 1. T-cell proliferation was quantitated by measuring 3H-thymidine uptake.
Results: Anti-SF-RI 1 antibodies of the IgA, IgE, IgG or IgM isotype were undetectable in all serum probes. SF-RI 1 did not stimulate T-cell proliferation in vitro. T-cells of patients treated with SF-RI 1 in vivo did not proliferate on incubation with SF-RI 1 in vitro.
Conclusion: The lack of demonstratable immunological reactions of preterm infants to bovine surfactant encourages the further clinical application of SF-RI 1.
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Bartmann, P., Bamberger, U., Pohlandt, F. et al. 4 IMMUNOLOGICAL REACTIONS TO BOVINE SURFAC- TANT IN PRETERM INFANTS. Pediatr Res 24, 261 (1988). https://doi.org/10.1203/00006450-198808000-00030
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DOI: https://doi.org/10.1203/00006450-198808000-00030
